Histology

The histology platform of the ORION technology center offers a whole range of equipment open to Collège de France staff and to university and external staff. It also provides training, advice and expertise in histology.

You are encountering a technical problem on a microscope ? Please call the :

1287

from any land-line phone of the institute.

We will do our best to help you out.

Cryostat NX70 (Room 316)

Cryostat for freezing sample. Cut of 5-100 µm.

Frozen microtome (Room 306b)

Freezing microtome for fixed sample. Cut of 10 -100 µm

Vibration microtome (Room 306a)

Vibration microtome for fixed or lived sample. Cut of 10-1500µm.

Paraffin Microtome (Room 306a)

Microtome for embedded samples (parrafin; resin). Cut of 2 - 500 µm

Dehydration machine - Citadelle (Room 305)

Tissue Dehydration Automaton is designed to prepare biological samples, chemical fixation to paraffin infiltration.

Paraffin embedding station - Histocentre 3 (Room 306 a)

The Thermo Scientific Histocentre 3 Center allow tissus embedding in paraffin

Laser Microdissection LMD-7000 (Room 316)

Laser microdissection allows the isolation of specific single cells or whole tissue areas.
It is generally used in genomics (DNA), transcriptomics (mRNA, miRNA) and proteomics.

Lenses :

-5x / 0,12 WD: 11,7mm
-10x / 0,3 WD: 11 mm
-20x / 0,4 WD: 6,9 mm PH1
-40x / 0,6 WD: 3,3-1,9 mm PH2
-63x / 0,7 WD: 2,6-1,8 PH2
-150x / 0,9 WD: 0,25mm

Filters :

- DAPI

- CFP

- GFP

- CY3

- CY5

- BH/PH

Cameras :

-DFC 360 FX / CCD / pixel size: 6,45*6,45 µm
-DFC 310 FX / CCD color / pixel size: 6,45*6,45 µm

Slide Scanner Axioscan 7 (Room 319)

The slide scanner allows the scanning of high-resolution histological sections, in brightfield, polarised light and fluorescence, in order to obtain an overview of the biological tissue.

Lenses :

-5x / 0,15 WD: 12 mm
-10x / 0,45 WD: 2,1 mm
-20x / 0,8 WD: 0,55 mm
-40x / 0,95 WD: 0,25 mm

Fluorescent filters :

-DAPI
-CFP
-GFP
-YFP
-CY3
-CY5
-CY7