The Orion technological core provide expertise and technical support for cell sorting and analysis. The specification of the cytometers is as follow :

FACS ARIA II (Becton Dickinson)

This cell sorter is equipped with :

  • 3 lasers (405nm, 488nm, 633nm)
  • 11 detectors : 2 morphological parameters (FSC, SSC) and 9 fluorescence parameters 

For the 488nm laser-line : 5 detectors (fluorescence filters : 530/30,585/42, 616/23, 695/40, 780/60)

For the 405nm laser-line  : 2 detectors (fluorescence filters : 450/50, 530/30)

For the 633nm laser-line  : 2 detectors (fluorescence filters : 660/20, 780/60)

  • 3 nozzle sizes : 70µm, 85µm and 100µm.
  • ACDU module (sorting in plates)

Cytoflex LX (Beckman Coulter)

This analyzer is equipped with 6 lasers and 23 detectors (2 morphological and 21 fluorescence parameters) :

For the 355nm laser line : 3 detectors (fluorescence filters : 400/30, 450/45, 675/30)

For the 405nm laser line : 5 detectors (fluorescence filters : 450/45, 525/40, 610/20, 660/20, 763/43)

For the 488nm laser line : 3 detectors (fluorescence filters : 525/40, 610/20, 660/50)

For the 561nm laser line : 5 detectors (fluorescence filters : 585/42, 610/20, 675/30, 710/50, 763/42)

For the 638nm laser line : 3 detectors (fluorescence filters : 660/20, 712/25, 763/43)

and for the 808nm laser line : 2 detectors (fluorescence filters : 840/20, 885/40)

The cytoflex LX is equipped with a plate loader for the 96-well plates

FACS ARIA FUSION (Becton Dickinson) with Biosafety Cabinet

This cell sorter is equipped with :

  • 4 lasers (405nm, 488nm, 561nm, 633nm)
  • 2 morphological parameters (FSC, SSC) 
  • 16 fluorescence parameters

For the 405nm laser-line  : 6 detectors (fluorescence filters : 450/50, 525/50, 610/20, 660/20, 710/50, 780/60)

For the 488nm laser-line : 2 detectors (fluorescence filters : 530/30, 695/40)

For the 561nm laser-line : 5 detectors (fluorescence filters : 582/15, 610/20, 670/14, 710/50, 780/60)

For the 633nm laser-line  : 3 detectors (fluorescence filters : 670/30, 730/45, 780/60)

  • 3 nozzle sizes : 70µm, 85µm and 100µm.
  • ACDU module (sorting in plates)

Managed by:

Magali Fradet