The Orion technological core provide expertise and technical support for cell sorting and analysis. The specification of the cytometer is as follow :

FACS ARIA II (Becton Dickinson)

This cell sorter is equipped with :

  • 3 lasers (405nm, 488nm, 633nm)
  • 2 morphological parameter (FSC, SSC) 
  • 7 fluorescence parameter  

For the 488nm laser-line : 5 fluorescence set (fluorescence filters : 530/30,585/42, 616/23, 695/40, 780/60)

For the 405nm laser-line  : 2 fluorescence set (fluorescence filters : 450/50, 530/30)

For the 633nm laser-line  : 2 fluorescence set (fluorescence filters : 660/20, 780/60)

  • 3 nozzle size : 70µm, 85µm and 100µm.
  • ACDU module (sorting in plates)

Cytoflex LX (Beckman Coulter)

This analyzer is equipped with 6 lasers :

– For the 355nm laser line : 3 parameters (400/30, 450/45, 675/30)

– For the 405nm laser line : 5 parameters (450/45, 525/40, 610/20, 660/20, 763/43)

– For the 488nm laser line : 3 parameters (525/40, 610/20, 660/50)

– For the 561nm laser line : 5 parameters, (585/42, 610/20, 675/30, 710/50, 763/42)

– For the 638nm laser line : 3 parameters (660/20, 712/25, 763/43)

– and for the 808nm laser line : 2 parameters (840/20, 885/40)

The cytoflex LX is equipped with a plate loader for the 96-well plates.

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