Cytometry

The Orion technological core provide expertise and technical support for cell sorting and analysis. The specification of the cytometer is as follow :

FACS ARIA II (Becton Dickinson)

  • 3 lasers (405nm, 488nm, 633nm)
  • 2 morphological parameter (FSC, SSC) 
  • 7 fluorescence parameter  :

For the 488nm laser-line : 5 fluorescence set (fluorescence filters : 530/30,585/42, 616/23, 695/40, 780/60)

For the 405nm laser-line  : 2 fluorescence set (fluorescence filters : 450/50, 530/30)

For the 633nm laser-line  : 2 fluorescence set (fluorescence filters : 660/20, 780/60)

  • 3 nozzle size : 70µm, 85µm and 100µm.
  • ACDU module (sorting in plates)