The histology platform of the ORION technology center offers a whole range of equipment open to Collège de France staff and to university and external staff. It also provides training, advice and expertise in histology.
To contact the histology facility, send an email to :
- All
- Cutting Machine
- Paraffin Machine
- Laser microdissection
- Slide scanner
Cryostat NX70 (Room 316)
Cryostat for freezing sample. Cut of 5-100 µm.
Freezing microtome (Room 306b)
Freezing microtome for fixed sample. Cut of 10 -100 µm
Vibrating microtome (Room 306a)
Vibrating microtome for fixed or lived sample. Cut of 10-1500µm.
Paraffin microtome (Room 306a)
Microtome for embedded samples (parrafin; resin). Cut of 2 – 500 µm
Citadel - Dehydration tissue processor (Room 305)
Dehydration tissue processor is designed to prepare biological samples, chemical fixation to paraffin infiltration.
Paraffin embedding station - Histocentre 3 (Room 306 a)
Thermo Scientific Histocentre 3 Center allows tissues embedding in paraffin
Laser Microdissection LMD7000 (Room 316)
Laser microdissection allows the isolation of specific single cells or whole tissue areas.
It is generally used in genomics (DNA), transcriptomics (mRNA, miRNA) and proteomics.
Lenses :
-5x / 0,12 WD: 11,7mm
-10x / 0,3 WD: 11 mm
-20x / 0,4 WD: 6,9 mm PH1
-40x / 0,6 WD: 3,3-1,9 mm PH2
-63x / 0,7 WD: 2,6-1,8 PH2
-150x / 0,9 WD: 0,25mm
Filters :
– DAPI
– CFP
– GFP
– CY3
– CY5
– BH/PH
Cameras :
-DFC 360 FX / CCD / pixel size: 6,45*6,45 µm
-DFC 310 FX / CCD color / pixel size: 6,45*6,45 µm
Slide Scanner Axioscan 7 (Room 319)
The slide scanner allows the scanning of high-resolution histological sections, in brightfield, polarised light and fluorescence, in order to obtain an overview of the biological tissue.
Lenses :
-5x / 0,15 WD: 12 mm
-10x / 0,45 WD: 2,1 mm
-20x / 0,8 WD: 0,55 mm
-40x / 0,95 WD: 0,25 mm
Fluorescent filters :
-DAPI
-CFP
-GFP
-CY3
-mCherry
-CY5
-CY7
Cameras :
-Axiocam 705 color / CMOS / 3,45*3,45 µm
-Axiocam 712 mono / CMOS / 3,45*3,45 µm
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