LSM980 troubleshooting
- Switch off the Zen software
- Switch off the multisocket
- Switch on the multisocket
- Start the Zen software
- Check the lasers are activated
- The laser key (below the microscope) must be turn toward “Laser”
- Check the fluorescent lamp
- Switch on the shutter of the fluorescent lamp, a blue light indicates it is ON
- If there is no blue light or if the shutter doesn’t answer, restart the fluorescent lamp
- If the restart of the lamp doesn’t help, please contact a member of the imaging facility
- If there is no blue light or if the shutter doesn’t answer, restart the fluorescent lamp
- Switch on the shutter of the fluorescent lamp, a blue light indicates it is ON
- On the touchpad, you have to check :
- The filter configuration selected in “Reflector” (i.e. “GFP” must be highlighted)
- The power of the excitation wavelength must be above 5%
- The shutter “RL Illumination” must be on “On”
- Check the black button right below the oculars
- It should be oriented toward the opened eyes
- Remove and clean the slide
- Clean the objective
- Add immersion medium on the objective (if oil objective)
- Put it back on focus
- The lines come from the bubbling system
- Move away the bubbling system from the microscope
- The lines don’t come from the bubbling system
- There might be a scanner malfunction. Restart the microscope to solve it.
- Readjust the position of the sample holder
- Use the jaws to tighten the slide
- Make sure the screen and the computer are on, and use the buttons at the back to select the input source of signal
- Force the computer to shut it down (hold the button for few seconds), wait 10 seconds, and restart the computer.