Axioscan 7 troubleshooting
- Increase the number of support point
- Click on “Adapt Selected Profil”
- Step 6 “Fine Focus”
- In “Onion Skin”, increase the parameter at 0.2
- Click on “Finish”
- Redo the Autofocus (AF)
- Click on “Adapt Selected Profil”
- In Step 5 then Step 6, click on “Live”, then click on “AF”
- Click on “Finish”
- If it doesn’t work, increase the range of the Z-stack at the step 6 and redo the autofocus
- The slice might be too thick, please check with a member of the facility.
- Define your channel of interest as reference for the focus point in Step 6 “Fine Focus”, by right-clicking on the channel of interest and clicking on “Define as reference”
- Change the detection thershold
- Click on “Check Profile”
- Change the threshold by moving the white boundary of the histogram
- If the automatic detection is impossible, switch to manual mode and draw around your slices using the ZEN drawing tools, then click on “Sort” to define the order of acquisition
- Change the reference channel used for stitching
- Click on “Adapt Selected Profile”
- In Step 7, right click on another channel and define it as reference
- Click on “New” to change the profile and start from zero
- Click on “Preview” to adapt and modify the previous profile