Spinning-disk X1 troubleshooting

  • Switch off the Zen software
  • Switch off the multisocket
  • Switch on the multisocket
  • Start the Zen software
  • No error message is visible on the controller
    • Check the labelling of the slider located on the right side of the microscope, below the objective (it should be a white label with “VIDEO” written on it.

    •  If not, please contact a member of the imaging facility for guidelines on how to change it.
  • An error message is visible on the controller
    • Switch off the Zen software
    • Switch off the two multisocket
    • Switch on the two multisocket
    • Restart the Zen software
  • On the touchpad, you have to check :
    • The filter configuration selected in “Reflector” (i.e. “GFP” must be highlighted)
    • The excitation wavelength set in “LED” (i.e. “B” must be highlighted)
    • The power of the excitation wavelength must be above 5%
    • The shutter “RL Illumination” must be on “On”
  • Remove and clean the slide
  • Clean the objective
  • Add immersion medium on the objective (if oil objective)
  • Put it back on focus
  • Ensure the objective you’re using is compatible with the Phase imaging
    • if not, switch to another modality (DIC or BF)
  • Do not use the well on the edge of the multiwell plate, as the objective can’t reach them